![]() The Col-0 plant was transformed by the floral dip method. We expressed the AIL1-SRDX repressor in the LAP1oe background ( SI Appendix, Fig. ![]() Molecular phenotypes include altered epidermal cell patterning and altered cell-cycle gene expression compared to wildtype.ĪtTCP23 coding sequence without stop codon was fused to the SRDX repressor domain (EAR peptide sequence LDLDLELRLGFA) with terminator codon TAA, and cloned into pK2GW7. We demonstrate here that the ERF-associated amphiphilic repression (EAR) motif repression domain (SRDX) can convert a transcriptional complex into a repressor via transrepression that is mediated by protein-protein interactions and show that transrepressive activity of SRDX can be used to detect such protein-protein interactions. To overcome this genetic redundancy, we generated a dominant repressor, antagonizing the function of endogenous AIL genes by expressing a fusion of AIL1 with an SRDX domain (a synthetic repressor) ( 44 ). Upward growth of the leaves and smaller rosette size plants have shorter pedicels with irregularly shaped larger sepals, shorter petals, and less trichomes compared to wildtype. Expression of AtTCP15SRDX, AtTCP15 fused with a SRDX repressor domain, induces extra endoredupli- cation in trichomes and cotyledon cells in transgenic. In CRES-T, chimeric repressors that are converted from the transcriptional activator to the repressor by fusion to SRDX, an inhibitory domain, are expressed.
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